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Research ArticleOriginal Research

Exploring Workplace Testing with Real-Time Polymerase Chain Reaction SARS-CoV-2 Testing

Lucas Fuentes, Nigam Shah, Sara Kelly, Glenn Harnett and Kevin A. Schulman
The Journal of the American Board of Family Medicine January 2022, 35 (1) 96-101; DOI: https://doi.org/10.3122/jabfm.2022.01.210284
Lucas Fuentes
From Department of Electrical Engineering, Stanford University, Stanford, CA (LF); Department of Biomedical Informatics, Stanford University, Stanford, CA (NS); Clinical Excellence Research Center, Stanford University, Stanford, CA (SK); Department of Medicine, Stanford University, Stanford, CA (KS).
M.Eng
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Nigam Shah
From Department of Electrical Engineering, Stanford University, Stanford, CA (LF); Department of Biomedical Informatics, Stanford University, Stanford, CA (NS); Clinical Excellence Research Center, Stanford University, Stanford, CA (SK); Department of Medicine, Stanford University, Stanford, CA (KS).
MBBS
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Sara Kelly
From Department of Electrical Engineering, Stanford University, Stanford, CA (LF); Department of Biomedical Informatics, Stanford University, Stanford, CA (NS); Clinical Excellence Research Center, Stanford University, Stanford, CA (SK); Department of Medicine, Stanford University, Stanford, CA (KS).
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Glenn Harnett
From Department of Electrical Engineering, Stanford University, Stanford, CA (LF); Department of Biomedical Informatics, Stanford University, Stanford, CA (NS); Clinical Excellence Research Center, Stanford University, Stanford, CA (SK); Department of Medicine, Stanford University, Stanford, CA (KS).
MD
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Kevin A. Schulman
From Department of Electrical Engineering, Stanford University, Stanford, CA (LF); Department of Biomedical Informatics, Stanford University, Stanford, CA (NS); Clinical Excellence Research Center, Stanford University, Stanford, CA (SK); Department of Medicine, Stanford University, Stanford, CA (KS).
MD
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    Figure 1.

    Specimen collection and testing workflow. Abbreviations: HCP, health care provider; POC, point-of-care; RT-PCR, real-time polymerase chain reaction.

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    Figure 2.

    Distribution of turnaround times for both the point-of-care (POC) and the laboratory real-time polymerase chain reaction (RT-PCR) test. Analysis of 852 samples with valid test results on both the single-test POC and laboratory RT-PCR test (see discussion in the text).

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    Table 1.

    Comparison of Single-Test Point-of-Care Results with the Reference Laboratory Tests

    Laboratory PositiveLaboratory Negative
    POC positive111Positive predictive value: 8.3%
    POC negative0840Negative predictive value: 100%
    Sensitivity: 100%Specificity: 98.7%
    • Note: Analysis of 852 samples with valid test results on both the single-test point-of-care (POC) and laboratory real-time polymerase chain reaction (RT-PCR) test (see discussion in the text). Laboratory RT-PCR testing served as the definition of disease in this analysis. Sensitivity is defined as the proportion of true-positive results among all positive results. Specificity is defined as the proportion of true-negative results among all negative results. Positive predictive value is the proportion of true-positive results among all positive test results. Negative predictive value is the proportion of true-negative results among all negative results.

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The Journal of the American Board of Family     Medicine: 35 (1)
The Journal of the American Board of Family Medicine
Vol. 35, Issue 1
January/February 2022
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Exploring Workplace Testing with Real-Time Polymerase Chain Reaction SARS-CoV-2 Testing
Lucas Fuentes, Nigam Shah, Sara Kelly, Glenn Harnett, Kevin A. Schulman
The Journal of the American Board of Family Medicine Jan 2022, 35 (1) 96-101; DOI: 10.3122/jabfm.2022.01.210284

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Exploring Workplace Testing with Real-Time Polymerase Chain Reaction SARS-CoV-2 Testing
Lucas Fuentes, Nigam Shah, Sara Kelly, Glenn Harnett, Kevin A. Schulman
The Journal of the American Board of Family Medicine Jan 2022, 35 (1) 96-101; DOI: 10.3122/jabfm.2022.01.210284
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