TY - JOUR T1 - Exploring Workplace Testing with Real-Time Polymerase Chain Reaction SARS-CoV-2 Testing JF - The Journal of the American Board of Family Medicine JO - J Am Board Fam Med SP - 96 LP - 101 DO - 10.3122/jabfm.2022.01.210284 VL - 35 IS - 1 AU - Lucas Fuentes AU - Nigam Shah AU - Sara Kelly AU - Glenn Harnett AU - Kevin A. Schulman Y1 - 2022/01/01 UR - http://www.jabfm.org/content/35/1/96.abstract N2 - Background: Molecular tests (ie, real-time polymerase chain reaction [RT-PCR]) and antigen tests are used to detect SARS-CoV-2. RT-PCR tests are generally considered to be the standard for clinical diagnosis of SARS-CoV-2 due to accuracy and reliability but can take longer to return results than antigen tests. Our aim was to examine if point-of-care (POC) testing for SARS-CoV-2 infection would provide a flexible resource to help achieve workplace safety. We compared test results and time-to-test results between a POC RT-PCR test and a send-out PCR test in a program implemented in summer 2020.Results: POC testing shortened the time to results to 110 minutes in the POC setting from the 754 minutes for send-out tests. The specificity of POC RT-PCR single POC testing was 98.7% compared with send-out RT-PCR testing and was confirmed at 99.8% in a validation analysis. The sensitivity of the POC testing was 100% compared with send-out RT-PCR, although in a validation analysis, sensitivity appeared as 0% because only the 12 positive or indeterminate samples on the first analysis were retested and the majority were false-positives that were correctly ruled out.Conclusions: POC testing for SARS-CoV-2 with RT-PCR technology is possible at reduced time compared with send-out PCR testing. ER -