Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses

…, W Haas, L Ting, MP Jedrychowski, JC Rogers… - Analytical …, 2012 - ACS Publications
Quantitative mass spectrometry methods offer near-comprehensive proteome coverage;
however, these methods still suffer with regards to sample throughput. Multiplex quantitation via …

TMTpro reagents: a set of isobaric labeling mass tags enables simultaneous proteome-wide measurements across 16 samples

…, K Kuhn, I Pike, RD Bomgarden, JC Rogers… - Nature …, 2020 - nature.com
Isobaric labeling empowers proteome-wide expression measurements simultaneously
across multiple samples. Here an expanded set of 16 isobaric reagents based on an isobutyl-…

[HTML][HTML] Plant cells contain two functionally distinct vacuolar compartments

N Paris, CM Stanley, RL Jones, JC Rogers - Cell, 1996 - cell.com
The plant cell vacuole has multiple functions, including storage of proteins and maintenance
of an acidic pH where proteases will have maximal activity. It has been assumed that these …

Medical student attitudes toward the doctor–patient relationship

…, L Hechtel, T Chang, E Tseng, JC Rogers - Medical …, 2002 - Wiley Online Library
Context Medical educators have emphasized the importance of teaching patient‐centred
care. Objectives To describe and quantify the attitudes of medical students towards patient‐…

[HTML][HTML] Molecular determinants of high affinity binding of α-scorpion toxin and sea anemone toxin in the S3-S4 extracellular loop in domain IV of the Na+ channel α …

JC Rogers, Y Qu, TN Tanada, T Scheuer… - Journal of Biological …, 1996 - ASBMB
α-Scorpion toxins and sea anemone toxins bind to a common extracellular site on the Na +
channel and inhibit fast inactivation. Basic amino acids of the toxins and domains I and IV of …

[HTML][HTML] Voltage sensor–trapping: enhanced activation of sodium channels by β-scorpion toxin bound to the S3–S4 loop in domain II

S Cestèle, Y Qu, JC Rogers, H Rochat, T Scheuer… - Neuron, 1998 - cell.com
Polypeptide neurotoxins alter ion channel gating by binding to extracellular receptor sites,
even though the voltage sensors are in their S4 transmembrane segments. By analysis of …

TMTpro-18plex: the expanded and complete set of TMTpro reagents for sample multiplexing

…, I Pike, K Kuhn, JC Rogers… - Journal of proteome …, 2021 - ACS Publications
The development of the TMTpro-16plex series expanded the breadth of commercial isobaric
tagging reagents by nearly 50% over classic TMT-11plex. In addition to the described …

cis-acting DNA elements responsive to gibberellin and its antagonist abscisic acid.

K Skriver, FL Olsen, JC Rogers… - Proceedings of the …, 1991 - National Acad Sciences
We have used a transient expression assay in aleurone protoplasts of barley to delineate
hormone response elements of the abscisic acid (ABA)-responsive rice gene Rab16A and of …

Tonoplast intrinsic protein isoforms as markers for vacuolar functions

GY Jauh, TE Phillips, JC Rogers - The Plant Cell, 1999 - academic.oup.com
Plant cell vacuoles may have storage or lytic functions, but biochemical markers specific for
the tonoplasts of functionally distinct vacuoles are poorly defined. Here, we use antipeptide …

Caleosins: Ca2+-binding proteins associated with lipid bodies

…, HB Nielsen, DJ Murphy, JC Rogers… - Plant molecular …, 2000 - Springer
We have previously identified a rice gene encoding a 27 kDa protein with a single Ca 2+ -binding
EF-hand and a putative membrane anchor. We report here similar genes termed …