Tuberculosis (TB) stigma is considered a vague concept, because it is not easily assessed. The purpose of this article is to review methodological approaches for assessing TB stigma. We reviewed the published studies and the gray literature. We also present the main features of a qualitative and quantitative assessment of TB stigma, which has been done in one specific project in Nicaragua. As a whole, we illustrate the variety of TB stigma in terms of domains, consequences, determinants and methods used; some features emerge more frequently than others from the studies. However, results of the review show a relative scarcity of the TB stigma assessment experiences, mainly in looking at the consequences for the TB patient and in the use of quantitative methods and scales. Additional assessment studies in diverse contexts are needed so that stigma will be considered a priority in the organization of care for people affected by tuberculosis.